In vitro assessment of some deodorant ingredients and determination of malodor inhibiting potentials of ascorbic acid, orlistat and mastic gum

Document Type : Original Article

Authors

1 Zagazig University Hospitals

2 Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt

Abstract

Axillary microbial flora has a role in sweat odor formation by transformation of
odorless natural secretions into volatile odorous molecules. From 190 axillary isolates
obtained from healthy volunteers, 97 isolates, representing three genera; Stahylococcus (39),
Micrococcus (13), Corynebacterium (45) were screened for their ability to produce
malodorous metabolites from testosterone oenanthate ester andhydroxyprogesterone caproate
by sensory, and chromatographic assessments. Only lipophilic Corynebacteriumspp(C.
jeikeium and C. macginleyi ) and Staphylococcus capitisisolates were able to metabolize
testosterone and hydroxy progesterone esters, with malodorous metabolites from the former
only.Quantification of the yield of conversion of substrates into metabolites by the action of
C. jeikeium was carried out using gas chromatography/mass spectrometryGC/MS. The
odourous metabolites are suspected to be Androstadienone and 5-α-dihydrotestosterone,
based on mass spectra and previously reported data. The effect of some selected material as
potential deodorants on such metabolic activities was studied. In the absence of inhibitor,
99.6% of testosterone ester substrate was converted into metabolites. Ascorbic acid and
orlistat dramatically inhibited testosterone ester metabolism allowing only 6.9 %, and 0.6
%conversion, respectively. While mastic gum allowed 84.3% conversion, no malodor was
observed. These three components have the potentials to be used as deodorants.

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