Precipitation of carboxymethyl cellulase enzyme biosynthesized by Trichoderma viride and Fusarium oxysporium was affected using organic solvents such as ethyl alcohol, acetone and butanol. Best results were obtained when 66% ethyl alcohol was used as a solvent. The precipitate was applied on a column chromatography with sephadex G-150 for fractionation and purification and the most active fractions were then collected. The optimal biochemical conditions under which the partially purified carboxy methyl cellulase (CMCase) exhibited its maximal activities were incubation temperature 50°C for 45 minutes at pH 5.0 in presence of 1% carboxy methyl cellulose. Zinc sulphate and calcium chloride were used as stimulators for the enzyme.
El-Sayed, M., El-Essawy, A., Ghaly, M., & Abd-El-Salam, S. (1994). BIOCHEMICAL STUDIES AND PARTIAL PURIFICATION OF CELLULASE ENZYME BIOSYNTHESIZED BY TRICHODERMA VIRIDE AND FUSARIUM OXYSPORIUM. Zagazig Journal of Pharmaceutical Sciences, 3(2), 69-77. doi: 10.21608/zjps.1994.186481
MLA
Mostafa El-Sayed; Abdel-Fatah El-Essawy; Mohamed Ghaly; Samia Abd-El-Salam. "BIOCHEMICAL STUDIES AND PARTIAL PURIFICATION OF CELLULASE ENZYME BIOSYNTHESIZED BY TRICHODERMA VIRIDE AND FUSARIUM OXYSPORIUM". Zagazig Journal of Pharmaceutical Sciences, 3, 2, 1994, 69-77. doi: 10.21608/zjps.1994.186481
HARVARD
El-Sayed, M., El-Essawy, A., Ghaly, M., Abd-El-Salam, S. (1994). 'BIOCHEMICAL STUDIES AND PARTIAL PURIFICATION OF CELLULASE ENZYME BIOSYNTHESIZED BY TRICHODERMA VIRIDE AND FUSARIUM OXYSPORIUM', Zagazig Journal of Pharmaceutical Sciences, 3(2), pp. 69-77. doi: 10.21608/zjps.1994.186481
VANCOUVER
El-Sayed, M., El-Essawy, A., Ghaly, M., Abd-El-Salam, S. BIOCHEMICAL STUDIES AND PARTIAL PURIFICATION OF CELLULASE ENZYME BIOSYNTHESIZED BY TRICHODERMA VIRIDE AND FUSARIUM OXYSPORIUM. Zagazig Journal of Pharmaceutical Sciences, 1994; 3(2): 69-77. doi: 10.21608/zjps.1994.186481
)
View on SCiNiTO