THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA

Timmis, Kenneth; Serry, Fathy; Abd El-Latif, Hemat; Chatwal, Gursharan; Shaker, Ghada

doi:10.21608/zjps.1995.186307

THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA

Document Type : Original Article

Authors

¹ Gesellschaft Für Biotechnologische Forschung (GBF), Braunschweig, Germany.

² Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt

³ Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt

⁴ University of Braunschweig, Germany

⁵ Department of Microbiology, Faculty of Pharmacy, Zagazig University, Egypt

10.21608/zjps.1995.186307

Abstract

The binding of IgA with C-protein was studied and compared with that of jacalin. SDS- PAGE and Western blotting for various human IgA forms (IgA₁, IgA₂, serum IgA and secretory IgA) proved the specificity of C-protein binding and the superiority of biotinylated C-protein over biotinylated jacalin as probing immunochemical reagent for IgA detection. The obtained results proved the usefulness of employing C-protein / sepharose columns in isolation of IgA from the whole serum. Although, a linear quantitative relationship was established with pure IgA, the development of ELISA technique for quantification of IgA in whole serum was not possible in the present study. However, a proposed procedure was set for further work.