EXPRESSION OF MOUSE ANTICREATINE KINASE (MAK 33), FAR FRAGMENT OF IgG ANTIBODY, IN THE METHYLOTROPHIC YEAST HANSENULA POLYMORPHA II. Gamma chain

Abdel-Salam, Hassan; Gatzke, Ralf; Serry, Fathy; Abd El-Latif, Hemat; Hollenberg, Cornelis

doi:10.21608/zjps.1996.185017

EXPRESSION OF MOUSE ANTICREATINE KINASE (MAK 33), FAR FRAGMENT OF IgG ANTIBODY, IN THE METHYLOTROPHIC YEAST HANSENULA POLYMORPHA II. Gamma chain

Document Type : Original Article

Authors

¹ Department of Microbiology, Faculty of Pharmacy, Zagazig University

² Institut für Mikrobiologie, Heinrich-Heine Universität, Düsseldorf, Universitätsstr. Düsseldorf, Germany.

³ Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt

⁴ Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt

10.21608/zjps.1996.185017

Abstract

The yeast Hapsenula pdymorpha was used as a host for expression of gamma chain peptide of F_Ab fragment of mouse articreatine kinase-M, IgG antibody. Two expression systems were designed and assembled based on the promoter codon of either of the preferentially highly expressed enzymes, formate dehydrogenase (FMD) and methanol oxidase (MOX) of H. polymorpha. Two expression plasmid vectors, PGAGU and PHAAI, with FMD and MOX promoters, representity, were contsructed and transformed into H. pdymorpha LR9 Expression of gamma chain gene yielded about 400 mg/L and 550 mg/L, respectively 7% and 10% of the total cell protein under the control of FMD and MOX promoters, respectively. The prepro alpha was incompletely processed and most of the gamma chain peptide accumulated within the cells, especially under the control of FMD promoter.