QUANTITATIVE DETERMINATION OF OFLOXACIN AND CIPROFLOXACIN HYDROCHLORIDE IN THE PRESENCE OF SOME RELATED SUBSTANCES IN PHARMACEUTICAL PREPARATIONS USING HPLC

Document Type : Original Article

Authors

1 Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.

2 Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazige University, Zagazig, Egypt

3 Analytical Chem. Department, Faculty of Pharmacy, Cairo University, Egypt

Abstract

An analytical method for the determination of ofloxacin or ciprofloxacin hydrochloride in pharmaceutical preparations by high performance liquid chromatography was developed. Piperazine, triethylformate, 3-chloro-4-fluoroaniline and 1,3-dichloro-4-fluorobenzene do not interfere with the analysis of ofloxacin. Also, cyclopropylamine, piperazine hexahydrate, ethylene diamine ciprofloxacin analog, 3- chloro-4- fluoroaniline, 1,3-dichloro-4-fluorobenzene and fluoroquinolonic acid do not Haterfere with the analysis of ciprofloxacin Salicylic acid was used as internal standard and the mobile phase consisted of a mixture of 35% methanol in 0.05 M sodium dihydrogen phosphate and the pH adjusted to 2.8 at flow rate 1 ml min-1.
The chromatographic separation was achieved on Lichrospher 60-RP column with ultraviolet detection at 274 nm. The BOcratic system was operated at ambient temperature and required 8 min of chromatographic time. The method was applied to Tanvid® tablets and Ciprinol ® ampoules with relative standard deviation 0.75 and 1.62, respectively.

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