PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS

Yousef, Nehal; Abd El-Latif, Hemat; Serry, Fathy; Cameron, J. A.

doi:10.21608/zjps.1998.184799

PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS

Document Type : Original Article

Authors

¹ Department of Microbiology, Faculty of Pharmacy, University of Zagazig - Egypt

² Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt

³ Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt

⁴ Department of Microbiology, Molecular and Cell Biology, Connecticut University , Storrs, U.S.A

10.21608/zjps.1998.184799

Abstract

In a previous screening study for polyhydroxy alkanoate (PHA) degrading activities, three isolates were obtained and characterized as Eikenella corrondens, Klebsiella pneumoniae and Alcaligenes faecalis . In the present study, PHA depolymerases from these three isolates were purified by DEAE anion exchange chromatography and by chromatofocusing ( isoelectric focusing ) after precipitation from the crude enzyme preparations by ammonium sulfate. The molecular weights for the three enzymes were determined by gel permeation sodium dodecyl sulphat (SDS) polyacrylamide gel eclectrophoresis.