DNA FINGERPRINTING AND KARYOTYPE OF CANDIDA ALBICANS ISOLATED FROM VAGINAL INFECTIONS

Document Type : Original Article

Authors

1 Department of Microbiology, Faculty of Pharmacy, University of Zagazig - Egypt

2 Department of Microbiology, Faculty of Medicine, Suez Canal University - Egypt

3 Department of Microbiology, Faculty of Medicine, University of Zagazig - Egypt

Abstract

The vaginopathic potential and the intravaginal morphology of different Candida albicans strains isolated from 25 symptomatic cases suffering from vaginitis and from 25 asymptomatic cases were studied. During infection of symptomatic patients, the Candida albicans cells did not maintain their yeast morphology but gave rise to hyphal filaments and germ tube formation as well as protease production. Vaginal Candida albicans isolates taken from the asypmtomatic patients were found to have inability of protease production and germ tube formation. Candida albicans cells forming germ tube demonstrated the highest percentage of adherence to epithelial cells. DNA figerprinting and electrophoretic karyotype anal ysis of Candida albicans strains of different morphology and pathology proved to have different DNA bands pattern of fingerprint. The results suggest that protease production, germ tube formation and adherence to epithelial cells play an important role in virulence of Candida albicans.

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