SPECTROPHOTOMETRIC AND SPECTROFLUORIMETRIC DETERMINATION OF AMINEPTINE AND NORTRIPTYLINE THROUGH TERNARY COMPLEX FORMATION WITH EOSIN AND Cu (II)

Document Type : Original Article

Authors

1 Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University. Zagazig. Egypt.

2 Analytical Chemistry Department , Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.

3 National Organization for Drug Control and Research, Giza, Egypt.

Abstract

Two sensitive, spectrophotometric and spectrofluorimetric procedures are developed for the determination of two tricyclic antidepressant (amineptine hydrochloride and nortriptyline hydrochloride). Both methods are based on the formation of ternary complex between copper (II), eosin and the two cited drugs. Spectrophotometrically, the complex was estimated by two methods, the first method depends on the extraction of the ternary complex with chloroform. The complex showed an absorption maximum at 524 and 502 nm. for amineptine and nortriptyline, respectively. The second spectrophotometric method depends on the direct measurement of the complex after addition of sodium lauryl sulphate ( for amineptine hydrochloride) or methyl cellulose (for nortriptyline hydrochloride) as surfactant at pH 3.6. Under the optimum conditions, the ternary complexes showed an absorption maximum at 550 nm for amineptine and nortriptyline. The solution of the ternary complex obeyed Beer's law in concentration range 2-14 and 6 50 µg ml-1 with the first method, 0.8 8 and 1.6 9.6 µg ml-l with the second method for amineptine and nortriptyline, respectively. The proposed methods were applied to the determination of the two cited drugs in pharmaceutical tablets. A fluorescence quenching method for the determination of the two tricyclic antidepressant by forming this ternary complex was also investigated for the propose of enhancing the sensitivity of the determination.

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