SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF LISINOPRIL IN PHARMACEUTICAL FORMULATION

Document Type : Original Article

Authors

1 Analytical Chemistry Department , Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.

2 Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig. Egypt

3 Analytical Chemistry, Faculty of Pharmacy, Department of Zagazig University - Zagazig - Egypt

4 Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University. Zagazig. Egypt.

Abstract

Three simple and sensitive spectrophotometric methods for the determination of lisinopril in tablets are developed. The first method involves oxidation of lisinopril with 1-chlorobenzotriazole reagent (CBT) in strong alkaline medium followed by measuring the absorbance at 350 nm. The method obeys Beer\''s law over concentration range 8-64 µg ml-1 with mean recovery 99.28±1.088. The second method involves the reaction of the primary amino group of lisinopril with ninhydrin reagent producing a coloured product with absorbance maximum at 564 nm. The linearity range was found to be 4 - 20 µgml-1 with mean recovery 99.09±1.069.The third method is based on the coupling reaction with 1,2-naphthaquinone-4-sulphonate (NQS) to form a coloured product (ʎmax: 305). Regression analysis of a Beer''s plot showed good correlation in concentration 4-24 µg ml-1 with mean recovery 99.40± 0.891. The optimum experimental parameters for the reactions have been studied and the reactions mechanisms are discussed. The validity of the described procedures was assessed. Statistical analysis of the results has been carried out revealing high accuracy and good precision. The suggested procedures could be used for the determination of lisinopril in tablets. The procedures were rapid, simple and sensitive for quality control application.

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