Phenolic constituents from the leaves of Tylophora indica and their antioxidant activities

Document Type : Original Article

Authors

Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt

Abstract

Four flavonoids; kaempferol-3-O-galactoside (trifolin) [1], quercetin-3-O-galactoside (hyperoside) [2], quercetin-3-O- glucoside (isoquercitrin) [3] and kaempferol-3-O-rutinoside [4] together with chlorogenic acid [5] and chlorogenic acid methyl ester [6] have been isolated and purified for the first time from the leaves of Tylophora indica by HPLC. The structures were elucidated by the application of MS, IR, UV, ID- and 2D-NMR spectroscopic analyses and by comparison with literature data. All compounds 1-6 were found to exhibit antioxidant effects, based on the scavenging activity of the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical.